生体分析化学研究室

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前田初男　Hatsuo MAEDA

【原著論文・総説】

(1)	Which are you watching, an individual ROS or total oxidative stress? H. Maeda Ann. N. Y. Acad. Sci., in press.

(2)	蛍光プローブで見たいものだけを見る－活性酸素種，酵素活性およびセレノプロテインを計る－前田初男蛋白質核酸酵素（増刊号「ケミカルバイオロジー」），2007, 52 (13), 1524-1528.

(3)	Design of a practical fluorescent probe for superoxide based on protection-deprotection chemistry of fluoresceins with benzenesulfonyl protecting groups H. Maeda, K. Yamamoto, I. Kohno, L. Hafsi, N. Itoh, S. Nakagawa, N. Kanagawa, K. Suzuki, and T. Uno. Chem. Eur. J., 2007, 13, 1946-1954.

(4)	3'-(2,4-Dinitrobenzenesulfonyl)-2',7'-dimethylfluorescein as a fuorescent probe for selenols H. Maeda, K. Katayama, H. Matsuno, and T. Uno Angew. Chem. Int. Ed., 2006, 45, 1810-1813.

(5)	Omics vs. Chemical Biology 前田初男生物工学，2006, 84, 169.

(6)	保護／脱保護化学に基づく蛍光プローブの設計開発前田初男生物工学，2006, 84, 184-187.

(7)	必須微量元素セレンをはかる：セレノールの可視化計測前田初男海洋化学研究，2006, 19, 34-40.

(8)	2,4-Dinitrobenzenesulfonyl fluoresceins as fluorescent alternatives to Ellman’s reagent in thiol-quantification enzyme assays H. Maeda, H. Matsuno, M. Ushida, K. Katayama, K. Saeki and N. Itoh Angew. Chem. Int. Ed., 2005, 44, 2922-2925.

(9)	Important role of the 3-mercaptopropionamide moiety in glutathione: Promoting effect on decomposition of the adduct of glutathione with the oxoammonium ion of TEMPO H. Maeda, H.-Y. Wu, Y. Yamauchi and H. Ohmori J. Org. Chem., 2005, 70, 8338-8343.

(10)	A design of fluorescent probes for superoxide based on a nonredox mechanism H. Maeda, K. Yamamoto, Y. Nomura, I. Kohno, L. Hafsi, N. Ueda, S. Yoshida, M. Fukuda, Y. Fukuyasu, Y. Yamauchi and N. Itoh J. Am. Chem. Soc., 2005, 127, 68-69.

(11)	化学的脱保護反応を発蛍光機構とする蛍光プローブの設計開発前田初男和光純薬時報，2005, 73, 2-4.

(12)	High performance liquid chromatography equipped with a cathodic detector and column-switching device as a high-throughput method for a phosphatase assay with p-nitrophenylphosphate Y. Yamauchi, M. Ido and H. Maeda J. Chromatogr. A, 2005, 1066, 127-132.

(13)	Fluorescent probes for hydrogen peroxide based on a non-oxidative mechanism H. Maeda, Y. Fukuyasu, S. Yoshida, M. Fukuda, K. Saeki, H. Matsuno, Y. Yamauchi, K. Yoshida, K. Hirata and K. Miyamoto Angew. Chem. Int. Ed., 2004, 43, 2389-2391.

(14)	High performance liquid chromatography with an electrochemical detector in the cathodic mode as a tool for the determination of p-nitro-phenol and assay of acid phosphatase in urine samples Y. Yamauchi, M. Ido, M. Ohta and H. Maeda Chem. Pharm. Bull., 2004, 52, 552-555.

【著書】

(1)	ベーシック薬学教科書シリーズ　分析科学萩中淳編，片岡洋行，小林典裕，田和理市，前田初男著，化学同人，2007．

(2)	第４章「酸・塩基の電離平衡，中和滴定」パートナー分析化学Ⅰ，齋藤寛，千熊正彦，山口政俊，萩中淳編，南光堂、2007，pp 31-64．

(3)	第１１章「電気化学」スタンダード薬学シリーズ２・物理系薬学Ⅰ．物質の物理的性質，日本薬学会編，東京化学同人，2005，pp 251-262.

(4)	第Ⅱ章「酸・塩基の電離平衡」 INTEGRATED ESEENTIALS 分析化学Ⅰ（改訂第５版），齋藤寛，千熊正彦，山口政俊編，南光堂、2002，pp 9-48．

【特許】

(1)	特願 2003-273659 (2003)，特開 2005-47898 (2005). 「スルホン酸エステルおよびそれを用いた蛍光プローブ」出願人：財団法人大阪産業振興機構発明者：前田初男，平田收正，宮本一久

(2)	特願 2003-273660 (2003)，特開 2005-47891 (2005). 「スルホン酸エステルおよびそれを用いた蛍光プローブ」出願人：財団法人大阪産業振興機構発明者：前田初男，伊藤徳夫

芝崎誠司　Seiji SHIBASAKI

【原著論文・総説】

(1)	Ito, J, Ebe, T,　Shibasaki, S, Fukuda, H, Kondo, A. Production of alkyl glucoside from cellooligosaccharides using yeast strains displaying Aspergillus aculeatus -glucosidase 1 J. Mol. Catal.B: Enzym. in press.

(2)	Fukuda, N, Ishii, J, Shibasaki, S, Ueda, M, Fukuda, M, Kondo, A. High-efficiency recovery of target cells using improved yeast display system for detection of 　　 protein-protein interactions. Appl Microbiol Biotechnol. 2007. 76(1):151-158.

(3)	Shibasaki, S, Kawabata, A, Ishii, J, Yagi, S, Kadonosono, T, Kato, M, Fukuda, N, Kondo, A, Ueda, M. Construction of a novel synergistic system for production and recovery of secreted recombinant proteins by the cell surface engineering. Appl Microbiol Biotechnol. 2007. 75(4):821-828.

(4)	Shibasaki, S, Kuroda, K, Duc Nguyen H, Mori, T, Zou W, Ueda, M. Detection of protein-protein interactions by a combination of a novel cytoplasmic membrane targeting system of recombinant proteins and fluorescence resonance energy transfer. Appl Microbiol Biotechnol. 2006. 70(4):451-457.

(5)	Kimura, Y, Shibasaki, S, Morisato, K, Ishizuka, N, Minakuchi, H, Nakanishi, K, Matsuo, M, Amachi, T, Ueda, M. and Ueda, K. Microanalysis for MDR1 ATPase by high-performance liquid chromatography with a titanium dioxide column. Anal. Biochem. 2004. 326(2):262-266.

(6)	Shibasaki, S, Tanaka, A. and Ueda, M. Development of combinatorial bioengineering using yeast cell surface display--order-made design of cell and protein for bio-monitoring. Biosens Bioelectron. 2003. 19(2): 123-130.

(7)	芝崎誠司、植田充美インテリジェントな細胞センサーバイオサイエンスとインダストリー　61(1): pp. 27-30.（2003）

(8)	Yasui, M, Shibasaki, S, Kuroda, K, Ueda, M, Kawada, N, Nishikawa, J, Nishihara T. and Tanaka, A. An arming yeast with the ability to entrap fluorescent 17beta-estradiol on the cell surface. 　　 Appl Microbiol Biotechnol. 2002. 59(2-3): 329-331.

(9)	Kuroda, K, Ueda, M, Shibasaki, S. and Tanaka, A. Cell surface-engineered yeast with ability to bind, and self-aggregate in response to, copper ion. Appl Microbiol Biotechnol. 2002. 59(2-3):259-264.

(10)	Shibasaki, S., Ueda, M., and Tanaka, A. Construction of Surface-Engineered Arming Yeasts with Fluorescent Protein Sensors in Response to Environmental Changes. Biological System Engineering. American Chemical Society, Washington, D.C., USA. 2002.　pp. 234-247.

(11)

Shibasaki, S, Ninomiya, Y, Ueda, M, Iwahashi, M, Katsuragi, T, Tani, Y, Harashima, S. and Tanaka, A.
Intelligent yeast strains with the ability to self-monitor the concentrations of intra- and
extracellular phosphate or ammonium ion by emission of fluorescence from the cell surface.
Appl Microbiol Biotechnol. 2001. 57(5-6): 702-707.

(12)	Kuroda, K, Shibasaki, S, Ueda, M. and Tanaka, A. Cell surface-engineered yeast displaying a histidine oligopeptide (hexa-His) has enhanced adsorption of and tolerance to heavy metal ions. Appl Microbiol Biotechnol. 2001. 57(5-6): 697-701.

(13)	Shibasaki, S, Ueda, M, Ye, K, Shimizu, K, Kamasawa, N, Osumi, M. and Tanaka, A. Creation of cell surface-engineered yeast that display different fluorescent proteins in 　　 response to the glucose concentration. Appl Microbiol Biotechnol. 2001. 57(4): 528-533.

(14)	Nakamura, Y, Shibasaki, S, Ueda, M, Tanaka, A, Fukuda, H. and Kondo, A. Development of novel whole-cell immunoadsorbents by yeast surface display of the IgG-binding domain. Appl Microbiol Biotechnol. 2001. 57(4): 500-505.

(15)

Shibasaki, S, Ueda, M, Iizuka, T, Hirayama, M, Ikeda, Y, Kamasawa, N, Osumi, M. and Tanaka, A.
Quantitative evaluation of the enhanced green fluorescent protein displayed on the cell surface of Saccharomyces cerevisiae by fluorometric and confocal laser scanning microscopic analyses.
Appl Microbiol Biotechnol. 2001. 55(4): 471-475.

(16)	Shibasaki, Y, Kamasawa, N, Shibasaki, S, Zou, W, Murai, T, Ueda, M, Tanaka, A. and Osumi, M. Cytochemical evaluation of localization and secretion of a heterologous enzyme displayed on yeast cell surface. FEMS Microbiol Lett. 2000. 192(2):243-248.

(17)	Ye, K, Shibasaki, S, Ueda, M, Murai, T, Kamasawa, N, Osumi, M, Shimizu, K. and Tanaka, A. Construction of an engineered yeast with glucose-inducible emission of green fluorescence from the cell surface. Appl Microbiol Biotechnol. 2000. 54(1):90-96.

【著書】

(1)	芝崎誠司、植田充美第５章　試験管内抗体作製と回収システム抗体医薬の最前線（シーエムシー出版） pp.46-55. (2007)

(2)	芝崎誠司、植田充美バイオ機器　シングルセルセンサーバイオテクノロジー総覧（通産資料出版会）　pp.671-676.（2005）

(3)	芝崎誠司、植田充美表層蛍光シグナルを用いたバイオセンシングのコンビナトリアルな展開コンビナトリアル・バイオテクノロジーの最前線（シーエムシー出版） pp.120-129.（2004）

塚本効司　Koji TSUKAMOTO

【原著論文】

(1)	Calix[6]arene-Based Template for X-ray Crystallographic Analysis of Template-Assembled Synthetic Proteins K. Tsukamoto, H. Ohishi, N. Maezaki, T. Tanaka, T. Ishida ChemBioChem, 2006, 7, 1559-1562.

(2)	Self-assembled octameric cage constructed by the potassium salt of p-tert-butylcalix[6]arene p-bromophenylalanine derivative in the solid state K. Tsukamoto, H. Ohishi, Y. Hiyama, N. Maezaki, T. Tanaka, T. Ishida Chem. Commun., 2006, 3606-3608.

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